Work Experiences

Mass Spectrometry and Chromatography Engineer
URMITE/Assistance Publique des Hôpitaux de Marseille (Méditerranée Infection), Marseille, France
From January 2011

Responsible of Proteomics and Metabolomics Analyses : projects organization and analysis management with the platform team.

  • Headspace-GC-MS (Perkin Elmer): identification and quantification of compounds in various biological samples (methane, ethanol, amino acids, pesticides, fatty acids …).
  • UPLC-QTOF (iClass + Vion Waters): known and unknow metabolite screening and quantification using Ion Mobility.
  • UHPLC-UV/FLUO : small chemicals detection and quantification for the hospital diagnosis (antibiotics in serum, LCR, urine) and the lab research projects (Short Chain Fatty Acids, insecticides, … in various biological samples).
  • nanoUPLC-QTOF-MS (nanoAcquity + Synapt G2Si Waters): identification of proteins in highly complex samples using 2D LC and ion mobility features. LC-MS data treatment with several algorithms: MASCOT 2.4 server (Matrix Science), Peaks DB (BSI) and PLGS (Waters). de novo sequencing of peptides. Enrolled in Research projects in the University of Medecine. Study of Intact Proteins.
  • MALDI-TOF-MS (Autoflex/Ultraflex Bruker)Peptide Mass Fingerprinting for the identification of proteins from in-gel digestion.  Use of  Biotools and Mascot software. Average masses measurements of  intact proteins.

Technical expert during calls for tender : PUMA HS-GC-MSQ-TOF Synapt (Waters quote), Q-TOF Vion.

In charge of the maintenance of all instruments.

Training of students and staff.

In charge of the Chemical Safety in the laboratory.

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Speaker – MS Day
Waters, Paris – October 2014 

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Jury Member
Aix-Marseille University – October 2014/November 2017

Stand-in teacher
Aix-Marseille University – from 2011 

  • Oral presentations in the field of Proteomics (Analytical chemistry MASTER’s students).
  • Oral presentations concerning chromatography and mass spectrometry analysis (Human pathogens MASTER’s students).

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Analytical Chemistry Project Manager
Avantage Nutrition Ltd, Marseille, France
September 2009 to December 2010 (15 months)

  • Analytical Chemistry Project Manager. Analysis of biologic samples: water and fat-soluble vitamins, carotenoids using LC-DAD (Agilent 1100) and fatty acid profiles (C6 to C24) using GC-FID (Clarus 500 Perkin Elmer). Technical maintenance of both chromatography systems. Operational Qualification and Performance Validation.
  • Responsible for the Security and Quality Management in the company. Drafting and updating of all the procedures and methods. Preparation of detailed estimates and order forms.

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Mass Spectrometry Engineer
Laboratory of Environmental Chemistry, UMR CNRS 5255, Bordeaux, France
September 2008 to August 2009 (12 months)

  • Analysis of small molecules and polymers using MALDI-TOF-MS (Autoflex I Bruker) and ESI QTRAP (Sciex) systems. Data treatment and preparation of analysis reports for clients and researchers.
  • MS optimisations (SIM and MRM) in order to reach high sensitivity for quantitation of pesticides, PAHs, VOCs. Use of the following systems: GC-MS (Agilent 7890A), GC-MS/MS (Waters Quattro micro GC), GC-TOF (Waters GCT Premier) and LC-MS/MS (Agilent 1200 LC System). Optimisation of selective extraction methods using SPME (Supelco) and SBSE (Twister GERSTEL).